Mouse VEGFR3/Flt-4 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB743G
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4
Tyr25-Asp770
Accession # P35917
Tyr25-Asp770
Accession # P35917
Specificity
Detects mouse VEGF R3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF R3, 10% cross-reactivity with rhVEGF R2, and 5% cross-reactivity with rhVEGF R1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse VEGFR3/Flt-4 Alexa Fluor® 488-conjugated Antibody
Detection of VEGF R3/Flt‑4 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt-4 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse VEGFR3/Flt-4 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line
Sample: bEnd.3 mouse endothelioma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGFR3/Flt-4
VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
References
- Finnerty, H. et al. (1993) Oncogene 8:2293.
- Joukov, V. et al. (1996) EMBO J. 15:290.
- Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 3
Alternate Names
Flt-4, FLT4, VEGF R3
Gene Symbol
FLT4
UniProt
Additional VEGFR3/Flt-4 Products
Product Specific Notices for Mouse VEGFR3/Flt-4 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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