Mouse VG5Q Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30481
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 368011
Product Specifications
Immunogen
E. coli-derived recombinant mouse VG5Q
Met1-Glu711
Accession # Q7TN31
Met1-Glu711
Accession # Q7TN31
Specificity
Detects mouse VG5Q in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse VG5Q Antibody
VG5Q in bEnd.3 Mouse Cell Line.
VG5Q was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse VG5Q Monoclonal Antibody (Catalog # MAB30481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse VG5Q Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line
Sample: Immersion fixed bEND.3 mouse endothelioma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VG5Q
Angiogenic Factor VG5Q, also known as AGGF1, is a presumably secreted protein expressed by vascular endothelial cells. Mouse VG5Q cDNA encodes a 714 aa precursor with a coiled coil domain, a forkhead (FHA) domain, and RNA-association domains (G-patch, OCRE). VG5Q binds the angiogenic TNF superfamily ligand TWEAK, and promotes endothelial cell proliferation. Human VG5Q mutations are associated with Klippel-Trenaunay syndrome, a congenital vascular morphogenesis disorder. Mouse VG5Q shares 78% aa identity with human VG5Q.
Long Name
Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1
Alternate Names
AGGF1
Entrez Gene IDs
55109 (Human)
Gene Symbol
AGGF1
UniProt
Additional VG5Q Products
Product Documents for Mouse VG5Q Antibody
Product Specific Notices for Mouse VG5Q Antibody
For research use only
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