Mouse Vitamin D BP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4188
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 884704
Product Summary for Mouse Vitamin D BP Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP
Met1-Ser476
Accession # P21614
Met1-Ser476
Accession # P21614
Specificity
Detects mouse Vitamin D BP in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Vitamin D BP Antibody
Mouse Vitamin D BP ELISA Standard Curve.
Recombinant Mouse Vitamin D BP protein was serially diluted 2-fold and captured by Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Mouse Vitamin D BP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Vitamin D BP DuoSet ELISA Kit (Catalog # DY4188-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vitamin D BP
VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.
Long Name
Vitamin D Binding Protein
Alternate Names
DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP
Gene Symbol
GC
UniProt
Additional Vitamin D BP Products
Product Documents for Mouse Vitamin D BP Antibody
Product Specific Notices for Mouse Vitamin D BP Antibody
For research use only
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