Mouse Vitamin D BP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4188
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
ELISA Capture, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP
Leu17-Ser476
Accession # P21614
Leu17-Ser476
Accession # P21614
Specificity
Detects mouse Vitamin D BP in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Vitamin D BP is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Vitamin D BP Antibody
Detection of Mouse Vitamin D BP by Western Blot.
Western blot shows lysates of mouse liver. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Vitamin D BP by Simple WesternTM.
Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Vitamin D BP at approximately 58 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse Vitamin D BP Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample:
Sample:
| Mouse liver tissue |
Western Blot
0.5 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vitamin D BP
VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.
Long Name
Vitamin D Binding Protein
Alternate Names
DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP
Gene Symbol
GC
UniProt
Additional Vitamin D BP Products
Product Documents for Mouse Vitamin D BP Antibody
Product Specific Notices for Mouse Vitamin D BP Antibody
For research use only
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