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Mouse VSIG2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4766

R&D Systems, part of Bio-Techne
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MAB4766
MAB4766-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 771625

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VSIG2
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109

Specificity

Detects mouse VSIG2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human VSIG2 or recombinant mouse VSIG1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse VSIG2 Antibody

VSIG2 antibody in Mouse Stomach by Immunohistochemistry (IHC-Fr).

VSIG2 in Mouse Stomach.

VSIG2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse VSIG2 Monoclonal Antibody (Catalog # MAB4766) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the plasma membranes of cells in gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse VSIG2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse stomach

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Alternate Names

CTH, CTXL

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Gene Symbol

VSIG2

UniProt

Additional VSIG2 Products

Product Documents for Mouse VSIG2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse VSIG2 Antibody

For research use only

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