Mouse XCL1/Lymphotactin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF486
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
ELISA Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Specificity
Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Lymphotactin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse XCL1/Lymphotactin Biotinylated Antibody
XCL1/Lymphotactin in Mouse Thymus.
XCL1/Lymphotactin was detected in perfusion fixed frozen sections of mouse thymus using Mouse XCL1/Lymphotactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF486) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse XCL1/Lymphotactin Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus
Sample: Perfusion fixed frozen sections of mouse thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Mouse XCL1/Lymphotactin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCL1/Lymphotactin
Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
References
- Kelner, G. et al. (1994) Science 266:1395.
- Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
Additional XCL1/Lymphotactin Products
Product Documents for Mouse XCL1/Lymphotactin Biotinylated Antibody
Product Specific Notices for Mouse XCL1/Lymphotactin Biotinylated Antibody
For research use only
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