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MPG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-870

Novus Biologicals, part of Bio-Techne
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NB300-870

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SVVDRVAEQDTQA corresponding to C-Terminus according to NP_002425.2, NP_001015052.1, NP_001015054.1.

Specificity

This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MPG Antibody

Western Blot: MPG Antibody [NB300-870]

Western Blot: MPG Antibody [NB300-870]

Western Blot: MPG Antibody [NB300-870] - HEK293 lysate (RIPA buffer, 35 ug total protein). Primary antibody at 1 ug/ml incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MPG Antibody [NB300-870]

Immunohistochemistry-Paraffin: MPG Antibody [NB300-870]

Immunohistochemistry-Paraffin: MPG Antibody [NB300-870] - Human uterus. Antibody at 5 ug/ml. Antigen retrieval with citrate buffer at pH 6. AP-staining.

Applications for MPG Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9 kDa band according to NP_002425.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MPG

MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.

Alternate Names

3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG, anpg, APNG, CRA36.1, CRA36.1 (3-methyl-adenine DNA glycosylase), DNA-3-methyladenine glycosylase, EC 3.2.2.21, MDGalkyladenine DNA glycosylase, MID1, N-methylpurine-DNA glycosylase, MPG, N-methylpurine-DNA glycosylasePIG16, PIG11, proliferation-inducing protein 11, proliferation-inducing protein 16

Entrez Gene IDs

4350 (Human)

Gene Symbol

MPG

Additional MPG Products

Product Documents for MPG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MPG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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