MPHOSPH10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84341

Novus Biologicals, part of Bio-Techne
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NBP1-84341
NBP1-84341-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MPHOSPH10 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDDLQENEDNKQHKESLKRVTFALPDDAETEDTGVLNVKKNSDEVKSSFEKRQEKMNEKIASLEKELLEKKPWQLQGEVTAQKRPENSLLEETLHFDHAVR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MPHOSPH10 Antibody

Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341]

Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341]

Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341] - Staining of human cerebral cortex, kidney, skin and testis using Anti-MPHOSPH10 antibody NBP1-84341 (A) shows similar protein distribution across tissues to independent antibody NBP2-38232 (B).
Immunocytochemistry/ Immunofluorescence: MPHOSPH10 Antibody [NBP1-84341]

Immunocytochemistry/ Immunofluorescence: MPHOSPH10 Antibody [NBP1-84341]

Immunocytochemistry/Immunofluorescence: MPHOSPH10 Antibody [NBP1-84341] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341]

Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341]

Immunohistochemistry-Paraffin: MPHOSPH10 Antibody [NBP1-84341] - Staining of human testis shows strong positivity in nucleoli in Leydig cells.
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Applications for MPHOSPH10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MPHOSPH10

MPHOSPH10 encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein is thought to be part of the U3 small nucleolar ribonucleoprotein complex, which is involved in rRNA processing.

Alternate Names

M phase phosphoprotein 10, M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein), MPP10CT90, MPP10P, U3 small nucleolar ribonucleoprotein protein MPP10

Gene Symbol

MPHOSPH10

Additional MPHOSPH10 Products

Product Documents for MPHOSPH10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MPHOSPH10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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