MPP3 Antibody (OTI8F1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72771

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8F1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MPP3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MPP3 (NP_001923) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MPP3 Antibody (OTI8F1) - Azide and BSA Free

Western Blot: MPP3 Antibody (OTI8F1)Azide and BSA Free [NBP2-72771]

Western Blot: MPP3 Antibody (OTI8F1)Azide and BSA Free [NBP2-72771]

Western Blot: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPP3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP3.
Immunocytochemistry/ Immunofluorescence: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771]

Immunocytochemistry/ Immunofluorescence: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771]

Immunocytochemistry/Immunofluorescence: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MPP3.
Flow Cytometry: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771]

Flow Cytometry: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771]

Flow Cytometry: MPP3 Antibody (OTI8F1) - Azide and BSA Free [NBP2-72771] - Analysis of Jurkat cells, using anti-MPP3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for MPP3 Antibody (OTI8F1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MPP3

MPP3 product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.

Alternate Names

Discs large homolog 3, discs, large homolog 3, DLG3MAGUK p55 subfamily member 3, membrane protein palmitoylated 3, membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3), Protein MPP3

Gene Symbol

MPP3

Additional MPP3 Products

Product Documents for MPP3 Antibody (OTI8F1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MPP3 Antibody (OTI8F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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