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MPPED2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80499

Novus Biologicals, part of Bio-Techne
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NBP1-80499

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the C terminal of human MPPED2. Peptide sequence PKLHVFGGIHEGYGIMTDGYTTYINASTCTVSFQPTNPPIIFDLPNPQGS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MPPED2 Antibody

Western Blot: MPPED2 Antibody [NBP1-80499]

Western Blot: MPPED2 Antibody [NBP1-80499]

Western Blot: MPPED2 Antibody [NBP1-80499] - MPPED2 reduces cell proliferation of breast carcinoma cell lines. qRT-PCR (left panel) performed in MDA-MB-231 and SKBR3 cells stably expressing MPPED2 or carrying the corresponding empty vector (EV). Data are reported as 2-delatadelataCt values +/- SD, compared to the EV, set equal to 1. Western blot (right panel) analysis confirming the expression of MPPED2. beta-actin was used to normalize the amount of loaded protein (left panel). Densitometric analysis was performed by using ImageJ software to evaluate MPPED2 protein expression compared to EV, set equal to 1. Original blots were shown in Supplementary Materials Figure S5. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2072-6694/11/6/797) licensed under a CC-BY license.
Western Blot: MPPED2 Antibody [NBP1-80499]

Western Blot: MPPED2 Antibody [NBP1-80499]

Western Blot: MPPED2 Antibody [NBP1-80499] - A204 cell lysate, concentration 0.2-1 ug/ml.

Applications for MPPED2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MPPED2

MPPED2 likely encodes a metallophosphoesterase. The encoded protein may play a role a brain development. Alternatively spliced transcript variants have been described. The unprocessed precursor is 294 amino acids in length and has a predicted molecular weight of 33.6KDa. It belongs to the UPF0046 familly and is predominantly expressed in fetal brain.

Alternate Names

239FBchromosome 11 open reading frame 8, C11orf8dJ1024C24.1, D11S302E, dJ873F21.1, EC 3.1, FAM1B, Fetal brain protein 239, metallophosphoesterase domain containing 2, Metallophosphoesterase domain-containing protein 2, metallophosphoesterase MPPED2

Gene Symbol

MPPED2

Additional MPPED2 Products

Product Documents for MPPED2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MPPED2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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