Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all MRCK Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1610 and 1660 of human myotonic dystrophy protein kinase-like alpha using the numbering given in entry CAH71185.1 (GeneID 8476).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRCK Antibody - BSA Free

Immunoprecipitation: MRCK Antibody [NBP1-46823]

Immunoprecipitation: MRCK Antibody [NBP1-46823]

Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MRCK antibody NBP1-46823 used for IP at 3 ug/mg lysate. MRCK was also immunoprecipitated by rabbit anti-MRCK antibody NBP1-46822, which recognizes an upstream epitope. For blotting immunoprecipitated MRC, NBP1-46822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: MRCK Antibody [NBP1-46823]

Immunoprecipitation: MRCK Antibody [NBP1-46823]

Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TORC3 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TORC3 was also immunoprecipitated by rabbit anti-TORC3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Applications for MRCK Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MRCK

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.

Alternate Names

CDC42 binding protein kinase alpha (DMPK-like), CDC42 binidng protein kinase beta, CDC42-binding protein kinase alpha, CDC42-binding protein kinase alpha (DMPK-like), DMPK-like alpha, EC 2.7.11, EC 2.7.11.1, KIAA0451DKFZp686L1738, MRCK, MRCK alpha, MRCKADKFZp686P1738, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Myotonic dystrophy protein kinase-like alpha, PK428FLJ23347, serine/threonine-protein kinase MRCK alpha, ser-thr protein kinase PK428, ser-thr protein kinase related to the myotonic dystrophy protein kinase

Gene Symbol

CDC42BPA

Additional MRCK Products

Product Documents for MRCK Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRCK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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