MRGPRF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83341

Novus Biologicals, part of Bio-Techne
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NBP1-83341
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MRGPRF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAGNCSWEAHPGNRNRMCPGLSEAPELYSRGFLTIEQIAMLP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRGPRF Antibody

Western Blot: MRGPRF Antibody [NBP1-83341]

Western Blot: MRGPRF Antibody [NBP1-83341]

Western Blot: MRGPRF Antibody [NBP1-83341] - Analysis in control (vector only transfected HEK293T lysate) and MRGPRF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MRGPRF Antibody [NBP1-83341]

Immunocytochemistry/ Immunofluorescence: MRGPRF Antibody [NBP1-83341]

Immunocytochemistry/Immunofluorescence: MRGPRF Antibody [NBP1-83341] - Staining of human cell line BJ shows localization to nuclear membrane & plasma membrane. Antibody staining shown in green.
Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341]

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341]

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 5 more images

Applications for MRGPRF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRGPRF

MRGF is an orphan receptor that may bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain.

Long Name

Mas-related G Protein-coupled Receptor Member F

Alternate Names

GPR140, GPR168, RTA

Gene Symbol

MRGPRF

Additional MRGPRF Products

Product Documents for MRGPRF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MRGPRF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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