MRP4/ABCC4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1471
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CNGQPSTLTIFETAL corresponding to C-Terminus according to NP_005836.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28785115). Rat reactivity reported in scientific literature (PMID: 22419695).
Localization
Cytoplasm
Specificity
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MRP4/ABCC4 Antibody
Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471]
Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for MRP4/ABCC4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
4 - 6 ug/mL
Immunohistochemistry-Paraffin
4 - 6 ug/mL
Peptide ELISA
Detection limit 1:1000
Western Blot
1-3 ug/ml
Application Notes
ICC/IF successful usage for human primary hepatocytes is reported in scientific literature (PMID: 20395535). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31669256).WB reactivity reported in (PMID: 25263207). Data obtained from a previous batch (different goat). WB: Approx. 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa band according to rat NP_596902.1 and 150 kDa band according to human NP_005836.2) is provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Data obtained from a previous batch (different goat). IHC-P: Human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MRP4/ABCC4
Long Name
Multidrug Resistance-associated Protein 4
Alternate Names
ABCC4, MOATB
Gene Symbol
ABCC4
UniProt
Additional MRP4/ABCC4 Products
Product Documents for MRP4/ABCC4 Antibody
Product Specific Notices for MRP4/ABCC4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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