MRRF Antibody (1D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00092399-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MRRF (NP_620132, 163 a.a. ~ 262 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG
Reactivity Notes
Human. Other species not tested.
Specificity
MRRF (1D3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for MRRF Antibody (1D3)
![Western Blot: MRRF Antibody (1D3) [H00092399-M01]](https://resources.bio-techne.com/images/products/MRRF-Antibody-1D3-Western-Blot-H00092399-M01-img0007.jpg "Western Blot: MRRF Antibody (1D3) [H00092399-M01]")
Western Blot: MRRF Antibody (1D3) [H00092399-M01]
Western Blot: MRRF Antibody (1D3) [H00092399-M01] - MRRF monoclonal antibody (M01), clone 1D3 Analysis of MRRF expression in A-431.![Immunohistochemistry-Paraffin: MRRF Antibody (1D3) [H00092399-M01]](https://resources.bio-techne.com/images/products/MRRF-Antibody-1D3-Immunohistochemistry-Paraffin-H00092399-M01-img0005.jpg "Immunohistochemistry-Paraffin: MRRF Antibody (1D3) [H00092399-M01]")
Immunohistochemistry-Paraffin: MRRF Antibody (1D3) [H00092399-M01]
Immunohistochemistry-Paraffin: MRRF Antibody (1D3) [H00092399-M01] - Analysis of monoclonal antibody to MRRF on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Sandwich ELISA: MRRF Antibody (1D3) [H00092399-M01] - Detection limit for recombinant GST tagged MRRF is approximately 0.03ng/ml as a capture antibody.
Applications for MRRF Antibody (1D3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MRRF
Alternate Names
mitochondrial, mitochondrial ribosome recycling factor, MTRRF
Entrez Gene IDs
92399 (Human)
Gene Symbol
MRRF
OMIM
604602 (Human)
UniProt
Additional MRRF Products
Product Documents for MRRF Antibody (1D3)
Product Specific Notices for MRRF Antibody (1D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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