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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D5

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 56-262 of human MRRF(NP_620132) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRRF Antibody (OTI1D5)

Western Blot: MRRF Antibody (OTI1D5) [NBP2-45434]

Western Blot: MRRF Antibody (OTI1D5) [NBP2-45434]

Western Blot: MRRF Antibody (1D5) [NBP2-45434] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRRF.
Immunohistochemistry: MRRF Antibody (OTI1D5) [NBP2-45434]

Immunohistochemistry: MRRF Antibody (OTI1D5) [NBP2-45434]

Immunohistochemistry: MRRF Antibody (1D5) [NBP2-45434] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: MRRF Antibody (OTI1D5) [NBP2-45434]

Immunohistochemistry: MRRF Antibody (OTI1D5) [NBP2-45434]

Immunohistochemistry: MRRF Antibody (1D5) [NBP2-45434] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for MRRF Antibody (OTI1D5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRRF

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. Mayincrease the efficiency of translation by recycling ribosomes from one round of translation to another

Alternate Names

mitochondrial, mitochondrial ribosome recycling factor, MTRRF

Gene Symbol

MRRF

Additional MRRF Products

Product Documents for MRRF Antibody (OTI1D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRRF Antibody (OTI1D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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