MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12043
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free [NBP3-12043]](https://resources.bio-techne.com/images/products/MS4A4A-Antibody-5C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12043-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free [NBP3-12043]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # 5C12
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.
Specificity
This antibody is specific for human extracellular MS4A4A. MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Scientific Data Images for MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free [NBP3-12043]
Immunocytochemistry/Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric [NBP3-12043] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 5C12 (NBP3-12043) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12043, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: MS4A4A
Long Name
Membrane-spanning 4-domains, Subfamily A, Member 4A
Alternate Names
4SPAN1, CD20L1, MS4A4, MS4A7
Gene Symbol
MS4A4A
Additional MS4A4A Products
Product Documents for MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free
Product Specific Notices for MS4A4A Antibody (5C12) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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