MSH2 Antibody (1B3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1767

Novus Biologicals, part of Bio-Techne
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NB100-1767
NB100-1767-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Monkey, Primate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B3

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all MSH2 Antibodies »

Product Summary for MSH2 Antibody (1B3) - BSA Free

Immunogen

Purified recombinant fragment of human MSH2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MSH2 Antibody (1B3) - BSA Free

Western Blot: MSH2 Antibody (1B3)BSA Free [NB100-1767]

Western Blot: MSH2 Antibody (1B3)BSA Free [NB100-1767]

Western Blot: MSH2 Antibody (1B3) [NB100-1767] - Analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunocytochemistry/Immunofluorescence: MSH2 Antibody (1B3) [NB100-1767] - Analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunohistochemistry-Paraffin: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunohistochemistry-Paraffin: MSH2 Antibody (1B3) [NB100-1767] - Analysis of human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

Applications for MSH2 Antibody (1B3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH2

MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the rapair of mismatch nucleotides following DNA replications or repair. Mutations in the NSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the intial cognition of mismatch nucleotides during the replication mismatch repair process.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Entrez Gene IDs

4436 (Human)

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (1B3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSH2 Antibody (1B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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