MSH2 Antibody (2L9I4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16692

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16692-100ul
NBP3-16692-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2L9I4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MSH2 Antibodies »

Product Summary for MSH2 Antibody (2L9I4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSH2 (P43246). MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MSH2 Antibody (2L9I4)

Western Blot: MSH2 Antibody (2L9I4) [NBP3-16692]

Western Blot: MSH2 Antibody (2L9I4) [NBP3-16692]

Western Blot: MSH2 Antibody (2L9I4) [NBP3-16692] - Western blot analysis of extracts of various cell lines, using MSH2 Rabbit mAb (NBP3-16692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692]

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692]

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692] - Immunohistochemistry of paraffin-embedded human colon using MSH2 Rabbit mAb (NBP3-16692) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692]

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692]

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692] - Immunohistochemistry of paraffin-embedded rat ovary using MSH2 Rabbit mAb (NBP3-16692) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for MSH2 Antibody (2L9I4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MSH2

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (2L9I4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MSH2 Antibody (2L9I4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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