MSH2 Antibody (MSH2/2622)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07211

Novus Biologicals, part of Bio-Techne
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NBP3-07211-100ug
NBP3-07211-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSH2/2622

Concentration

0.2 mg/ml

View all MSH2 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary) (Uniprot: IDP43246)

Localization

Nuclear

Marker

DNA Mismatch Repair Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08957)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MSH2 Antibody (MSH2/2622)

Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).
Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).
Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211]

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).
View 4 more images

Applications for MSH2 Antibody (MSH2/2622)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MSH2

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (MSH2/2622)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSH2 Antibody (MSH2/2622)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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