MSH3 Antibody (CL14064)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24588
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL14064
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was designed to target the recombinant protein MSH3, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for MSH3 Antibody (CL14064)
Immunohistochemistry-Paraffin: MSH3 Antibody (CL14064) [NBP3-24588]
Staining of human endometrium shows moderate nuclear positivity in glandular and in stromal cells.
Western Blot: MSH3 Antibody (CL14064) [NBP3-24588]
Western blot analysis in human cell line U-251 MG.
Immunohistochemistry-Paraffin: MSH3 Antibody (CL14064) [NBP3-24588]
Staining of human cervical cancer shows strong nuclear positivity in tumor cells.
Applications for MSH3 Antibody (CL14064)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MSH3
Alternate Names
Divergent upstream protein, DNA mismatch repair protein Msh3, DUC1, DUG, DUPMGC163306, hMSH3, Mismatch repair protein 1, MRP1MGC163308, mutS (E. coli) homolog 3, mutS homolog 3 (E. coli)
Gene Symbol
MSH3
Additional MSH3 Products
Product Documents for MSH3 Antibody (CL14064)
Product Specific Notices for MSH3 Antibody (CL14064)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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