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MSH6 Antibody (CL13844)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24582

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-24582-100ul
NBP3-24582-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13844

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MSH6 Antibody (CL13844)

Immunogen

This antibody was designed to target the recombinant protein MSH6, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MSH6 Antibody (CL13844)

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.

Applications for MSH6 Antibody (CL13844)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (CL13844)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MSH6 Antibody (CL13844)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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