MSH6 Antibody (MSH6/3086)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79712
![Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Flow-Cytometry-NBP2-79712-img0006.jpg "Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
![Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Western-Blot-NBP2-79712-img0005.jpg "Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # MSH6/3086
Concentration
0.2 mg/ml
Product Summary for MSH6 Antibody (MSH6/3086)
Immunogen
Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary) (Uniprot: P52701)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79846)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MSH6 Antibody (MSH6/3086)
Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Western Blot Analysis of HCT116 cell lysate using MSH6 Antibody (MSH6/3086).![Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Immunocytochemistry-Immunofluorescence-NBP2-79712-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Immunocytochemistry/Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with RedDot (Red).![Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Immunohistochemistry-Paraffin-NBP2-79712-img0003.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Antibody (MSH6/3086).![Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Immunohistochemistry-Paraffin-NBP2-79712-img0002.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
![Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.bio-techne.com/images/products/MSH6-Antibody-MSH6-3086-Protein-Array-NBP2-79712-img0004.jpg "Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Applications for MSH6 Antibody (MSH6/3086)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MSH6
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein
Gene Symbol
MSH6
UniProt
Additional MSH6 Products
Product Documents for MSH6 Antibody (MSH6/3086)
Product Specific Notices for MSH6 Antibody (MSH6/3086)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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