MST1/STK4 Antibody (01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06376

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 01

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MST1/STK4 Antibodies »

Product Summary for MST1/STK4 Antibody (01)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MST1/STK4 (Uniprot#: Q13043-1; Glu2-Phe487).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MST1/STK4 Antibody (01)

Immunocytochemistry/ Immunofluorescence: MST1/STK4 Antibody (01) [NBP3-06376]

Immunocytochemistry/ Immunofluorescence: MST1/STK4 Antibody (01) [NBP3-06376]

Immunocytochemistry/Immunofluorescence: MST1/STK4 Antibody (01) [NBP3-06376] - Immunofluorescence staining of Human STK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human STK4 monoclonal antibody (1:60) at 37 degrees celsius for 1 hour. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.
Flow Cytometry: MST1/STK4 Antibody (01) [NBP3-06376]

Flow Cytometry: MST1/STK4 Antibody (01) [NBP3-06376]

Flow Cytometry: MST1/STK4 Antibody (01) [NBP3-06376] - Flow cytometric analysis of Human STK4 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen (TM)), stained with purified anti-Human STK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for MST1/STK4 Antibody (01)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST1/STK4

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

Long Name

Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4

Alternate Names

KRS2, STK4, YSK3

Gene Symbol

STK4

Additional MST1/STK4 Products

Product Documents for MST1/STK4 Antibody (01)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MST1/STK4 Antibody (01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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