MT-ATP6 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05578
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 127-226 of mouse MT-ATP6 (NP_904333.1). IQAYVFTLLVSLYLHDNT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MT-ATP6 Antibody - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: MT-ATP6 Antibody - Azide and BSA Free [NBP3-05578]](https://resources.bio-techne.com/images/products/MT-ATP6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05578-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MT-ATP6 Antibody - Azide and BSA Free [NBP3-05578]")
Immunocytochemistry/ Immunofluorescence: MT-ATP6 Antibody - Azide and BSA Free [NBP3-05578]
Immunocytochemistry/Immunofluorescence: MT-ATP6 Antibody [NBP3-05578] - Immunofluorescence analysis of U2OS cells using MT-ATP6 antibody (NBP3-05578) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: MT-ATP6 Antibody [NBP3-05578] -
Western Blot: MT-ATP6 Antibody [NBP3-05578] - analysis of HepG2, using ATP6 Rabbit pAb at 1:2000；1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.Applications for MT-ATP6 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:100-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MT-ATP6
Alternate Names
ATP synthase F0 subunit 6, ATP6, ATPase subunit 6, ATPase6, MTATP6
Gene Symbol
ATP6
Additional MT-ATP6 Products
Product Documents for MT-ATP6 Antibody - Azide and BSA Free
Product Specific Notices for MT-ATP6 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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