MT-ND2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05589

Novus Biologicals, part of Bio-Techne
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NBP3-05589
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MT-ND2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MT-ND2 (YP_003024027.1). LLSLGGLPPLTGFLPKWAIIEEFTKNNSLIIPTIMATITLLNLYFYLRLIYSTSITLLPMSNNVKMKWQFEHTKPTPFLPTLIALTTLLLPISPFMLMIL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MT-ND2 Antibody - Azide and BSA Free

Western Blot: MT-ND2 AntibodyAzide and BSA Free [NBP3-05589]

Western Blot: MT-ND2 AntibodyAzide and BSA Free [NBP3-05589]

Western Blot: MT-ND2 Antibody [NBP3-05589] - Western blot analysis of extracts of various cell lines, using MT-ND2 antibody (NBP3-05589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: MT-ND2 Antibody - Azide and BSA Free [NBP3-05589]

Immunocytochemistry/ Immunofluorescence: MT-ND2 Antibody - Azide and BSA Free [NBP3-05589]

Immunocytochemistry/Immunofluorescence: MT-ND2 Antibody [NBP3-05589] - Immunofluorescence analysis of HeLa cells using MT-ND2 antibody (NBP3-05589) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MT-ND2 Antibody - Azide and BSA Free [NBP3-05589]

Immunohistochemistry-Paraffin: MT-ND2 Antibody - Azide and BSA Free [NBP3-05589]

Immunohistochemistry-Paraffin: MT-ND2 Antibody [NBP3-05589] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MT-ND2 Rabbit pAb (NBP3-05589) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for MT-ND2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MT-ND2

Alternate Names

MTND2, NADH dehydrogenase subunit 2, ND2

Gene Symbol

ND2

Additional MT-ND2 Products

Product Documents for MT-ND2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MT-ND2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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