MT-ND3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93832

Novus Biologicals, part of Bio-Techne
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NBP2-93832-0.02ml
NBP2-93832-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MT-ND3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-70 of human MT-ND3 (YP_003024033.1). MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MT-ND3 Antibody - Azide and BSA Free

Western Blot: MT-ND3 AntibodyAzide and BSA Free [NBP2-93832]

Western Blot: MT-ND3 AntibodyAzide and BSA Free [NBP2-93832]

Western Blot: MT-ND3 Antibody [NBP2-93832] - Analysis of extracts of various cell lines, using MT-ND3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: MT-ND3 Antibody - Azide and BSA Free [NBP2-93832]

Immunocytochemistry/ Immunofluorescence: MT-ND3 Antibody - Azide and BSA Free [NBP2-93832]

Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-93832] - Analysis of L929 cells using MT-ND3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MT-ND3 Antibody - Azide and BSA Free [NBP2-93832]

Immunohistochemistry-Paraffin: MT-ND3 Antibody - Azide and BSA Free [NBP2-93832]

Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Mouse kidney using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for MT-ND3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MT-ND3

Alternate Names

Complex I ND3 Subunit, EC 1.6.5.3, Mitochondrially Encoded NADH Dehydrogenase 3, MTND3, NADH Dehydrogenase 3, NADH Dehydrogenase Subunit 3, NADH Dehydrogenase, Subunit 3 (Complex I), NADH3, NADH-Ubiquinone Oxidoreductase Chain 3, ND3

Gene Symbol

ND3

Additional MT-ND3 Products

Product Documents for MT-ND3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MT-ND3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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