MTAP Antibody (2G4)
Novus Biologicals, part of Bio-Techne | Catalog # H00004507-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2G4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MTAP (AAH18625, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDSHVRRACFPFTFHHDCFQRPPQKPSRSHCVSCATCRTMS
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Cytoplasmic
Specificity
MTAP - methylthioadenosine phosphorylase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for MTAP Antibody (2G4)
Western Blot: MTAP Antibody (2G4) [H00004507-M01]
Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in NIH/3T3.Immunocytochemistry/ Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01]
Immunocytochemistry/Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01] - Analysis of monoclonal antibody to MTAP on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: MTAP Antibody (2G4) [H00004507-M01]
Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4 Analysis of MTAP expression in HeLa.Applications for MTAP Antibody (2G4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32284171).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MTAP
Long Name
5'-Methylthioadenosine Phosphorylase
Alternate Names
BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase
Entrez Gene IDs
4507 (Human)
Gene Symbol
MTAP
OMIM
156540 (Human)
UniProt
Additional MTAP Products
Product Documents for MTAP Antibody (2G4)
Product Specific Notices for MTAP Antibody (2G4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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