MTAP Antibody (MTAP/3137R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08965
Recombinant Monoclonal Antibody
![Western Blot: MTAP Antibody (MTAP/3137R)Azide and BSA Free [NBP3-08965]](https://resources.bio-techne.com/images/products/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Western-Blot-NBP3-08965-img0003.jpg "Western Blot: MTAP Antibody (MTAP/3137R)Azide and BSA Free [NBP3-08965]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # MTAP/3137R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for MTAP Antibody (MTAP/3137R) - Azide and BSA Free
Immunogen
Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary) (Uniprot: Q13126)
Localization
Cytoplasmic
Specificity
Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
Marker
Tumor Suppressor Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07220).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MTAP Antibody (MTAP/3137R) - Azide and BSA Free
Western Blot: MTAP Antibody (MTAP/3137R)Azide and BSA Free [NBP3-08965]
Western Blot: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]](https://resources.bio-techne.com/images/products/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08965-img0002.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]")
Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]
Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]](https://resources.bio-techne.com/images/products/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08965-img0001.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]")
Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965]
Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).Applications for MTAP Antibody (MTAP/3137R) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MTAP
Long Name
5'-Methylthioadenosine Phosphorylase
Alternate Names
BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase
Gene Symbol
MTAP
Additional MTAP Products
Product Documents for MTAP Antibody (MTAP/3137R) - Azide and BSA Free
Product Specific Notices for MTAP Antibody (MTAP/3137R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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