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MTBP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76747

Novus Biologicals, part of Bio-Techne
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NBP1-76747
NBP1-76747-0.025mg

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid peptide near the center of human MTBP. The immunogen is located within amino acids 110 - 160 of MTBP. Amino Acid Squence: GAVECFEEEDSNSRESLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTBP Antibody - BSA Free

Western Blot: MTBP AntibodyBSA Free [NBP1-76747]

Western Blot: MTBP AntibodyBSA Free [NBP1-76747]

Western Blot: MTBP Antibody [NBP1-76747] - Analysis of MTBP expression in K562 cell lysate with MTBP antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: MTBP Antibody - BSA Free [NBP1-76747]

Immunocytochemistry/ Immunofluorescence: MTBP Antibody - BSA Free [NBP1-76747]

Immunocytochemistry/Immunofluorescence: MTBP Antibody [NBP1-76747] - Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: MTBP Antibody - BSA Free [NBP1-76747]

Immunocytochemistry/ Immunofluorescence: MTBP Antibody - BSA Free [NBP1-76747]

Immunocytochemistry/Immunofluorescence: MTBP Antibody [NBP1-76747] - K562 cells with MTBP antibody at 5 ug/ml.

Applications for MTBP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Western Blot

1-2 ug/ml
Application Notes
A 104 kDa band can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTBP

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.

Alternate Names

hMTBP, MDM2 (mouse double minute 2)-binding protein, 104kD, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) bindingprotein, 104kDa, mdm2-binding protein, MDM2BP

Gene Symbol

MTBP

Additional MTBP Products

Product Documents for MTBP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MTBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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