MTERFD1 Antibody (A8-A10-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32611
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A8-A10-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein with Human MTERFD1 aa 29-417 / 417. (Uniprot: Q96E29)
Localization
Mitochondrion.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MTERFD1 Antibody (A8-A10-R)
Western Blot: MTERFD1 Antibody (A8-A10-R) [NBP3-32611]
Western Blot: MTERFD1 Antibody (A8-A10-R) [NBP3-32611] - Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (NBP3-32611) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: SK-OV-3 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 48 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32611) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for MTERFD1 Antibody (A8-A10-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MTERFD1
Alternate Names
CGI-12, FLJ10939, Mitochondrial transcription termination factor 3, MTERF domain containing 1, mTERF domain-containing protein 1, mitochondrial, mTERF3
Gene Symbol
MTERF3
Additional MTERFD1 Products
Product Documents for MTERFD1 Antibody (A8-A10-R)
Product Specific Notices for MTERFD1 Antibody (A8-A10-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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