MTF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86380
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse, Bovine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCG
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%). Mouse reactivity reported in scientific literature (PMID: 24529376). Bovine reactivity reported in scientific literature (PMID: 31068538).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MTF1 Antibody
![Western Blot: MTF1 Antibody [NBP1-86380]](https://resources.bio-techne.com/images/products/MTF1-Antibody-Western-Blot-NBP1-86380-img0010.jpg "Western Blot: MTF1 Antibody [NBP1-86380]")
Western Blot: MTF1 Antibody [NBP1-86380]
Western Blot: MTF1 Antibody [NBP1-86380] - Analysis in control (vector only transfected HEK293T lysate) and MTF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: MTF1 Antibody [NBP1-86380]](https://resources.bio-techne.com/images/products/MTF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86380-img0009.jpg "Immunocytochemistry/ Immunofluorescence: MTF1 Antibody [NBP1-86380]")
Immunocytochemistry/ Immunofluorescence: MTF1 Antibody [NBP1-86380]
Immunocytochemistry/Immunofluorescence: MTF1 Antibody [NBP1-86380] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.![Western Blot: MTF1 Antibody [NBP1-86380]](https://resources.bio-techne.com/images/products/MTF1-Antibody-Western-Blot-NBP1-86380-img0007.jpg "Western Blot: MTF1 Antibody [NBP1-86380]")
Western Blot: MTF1 Antibody [NBP1-86380]
Western Blot: MTF1 Antibody [NBP1-86380] - Analysis in human cell line HDLM-2.Applications for MTF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Frozen
Reported in scientific literature (PMID 24529376 ).
Immunohistochemistry-Paraffin
Reported in scientific literature (PMID: 24529376).
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MTF1
Alternate Names
metal regulatory transcription factor 1, metal-regulatory transcription factor 1, metal-responsive transcription factor 1, MGC23036, MRE-binding transcription factor, MRE-binding transcription factor-1, MTF-1, Transcription factor MTF-1, zinc regulatory factor, ZRF
Gene Symbol
MTF1
Additional MTF1 Products
Product Documents for MTF1 Antibody
Product Specific Notices for MTF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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