MTHFD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06054
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence RGDLNDCFIPCTPK corresponding to internal region according to NP_005947.2.
Epitope
RGDLNDCFIPCTPK
Marker
Cytoplasm Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MTHFD1 Antibody
Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] -
Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] -
Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] -
Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for MTHFD1 Antibody
Application
Recommended Usage
ELISA
Detection limit 1:1000
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
3.75 ug/ml
Western Blot
1 - 3 ug/ml
Application Notes
Approx. 100kDa band observed in lysates of cell lines Jurkat and MOLT4 and approx.110kDa in lysates of cell line MCF7 (calculated MW of 102kDa according to NP_005947.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MTHFD1
Alternate Names
C1-THF synthase, EC 6.3.4.3, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, MTHFCC-1-tetrahydrofolate synthase, cytoplasmic, MTHFDcytoplasmic C-1-tetrahydrofolate synthase
Gene Symbol
MTHFD1
UniProt
Additional MTHFD1 Products
Product Documents for MTHFD1 Antibody
Product Specific Notices for MTHFD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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