MTHFD1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78279

Novus Biologicals, part of Bio-Techne
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NBP1-78279
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MTHFD1L Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DTNDRFLRKITIGQ corresponding to internal region according to NP_056255.2.

Epitope

aa535-538

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MTHFD1L Antibody

Western Blot: MTHFD1L Antibody [NBP1-78279]

Western Blot: MTHFD1L Antibody [NBP1-78279]

Western Blot: MTHFD1L Antibody [NBP1-78279] - A. NIH3T3, B. Mouse Brain and C. Rat Brain lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP1-78279]

Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP1-78279]

Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP1-78279] - Human Tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: MTHFD1L Antibody [NBP1-78279]

Western Blot: MTHFD1L Antibody [NBP1-78279]

Western Blot: MTHFD1L Antibody [NBP1-78279] - Human Cer (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MTHFD1L Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 110 kDa band observed in human brain (cerebellum) (calculated MW of 106 kDa band according to NP_056255.2). The same band is observed in lysates of NIH3T3, mouse brain and rat brain.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTHFD1L

MTHFD1L, also known as Monofunctional C1-tetrahydrofolate synthase, mitochondrial, consists of 2 a 978 amino acid isoform1 that is 106 kDa and a 275 amino acid isoform 2 that is 30 kDa; has mitochondrion subcellular location and highest expression found in placenta, thymus and brain; it is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion which is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine, and also may provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. Current research is being performed on this protein involvement in cleft lip/palate, neural tube defect, homocysteine, coronary heart disease, chronic myeloid leukemia, Down syndrome, colon adenocarcinoma, nephropathy, Alzheimer's disease, dementia, colorectal cancer, leukemia, and atherosclerosis. This protein plays role in One carbon pool by folate and Metabolic pathways where it interacts with CASP4, MAGED1, WRAP73, WDR8, MAP1LC3A, and plus more than 40 other proteins.

Alternate Names

10-formyl-THF synthetase, dJ292B18.2, DKFZP586G1517, EC 6.3.4.3, FLJ21145, Formyltetrahydrofolate synthetase, formyltetrahydrofolate synthetase domain containing 1, FTHFSDC1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, mitochondrial C1-tetrahydrofolate synthase, mitochondrial C1-tetrahydrofolate synthetase, monofunctional C1-tetrahydrofolate synthase, mitochondrial, MTC1THFS

Entrez Gene IDs

270685 (Mouse); 361472 (Rat)

Gene Symbol

MTHFD1L

Additional MTHFD1L Products

Product Documents for MTHFD1L Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MTHFD1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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