MTHFR Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45222
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NRPTQNARETEAP corresponding to C-Terminus according to NP_005948.3.
Epitope
C-Terminus
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MTHFR Antibody
![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222]](https://resources.bio-techne.com/images/products/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP1-45222-img0001.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222]")
Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222]
Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for MTHFR Antibody
Application
Recommended Usage
Immunohistochemistry
3.75 ug/ml
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.2 ug
Application Notes
Western blot: Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.6kDa according to NP_005948.3. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen.
IHC: Paraffin embedded Human Spleen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MTHFR
Alternate Names
EC 1.5.1.20, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH)
Entrez Gene IDs
4524 (Human)
Gene Symbol
MTHFR
UniProt
Additional MTHFR Products
Product Documents for MTHFR Antibody
Product Specific Notices for MTHFR Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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