MTHFR Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27549
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Bovine (100%), Canine (100%), Human (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SRPKSYIYRTQEWDE corresponding to internal region according to NP_005948.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MTHFR Antibody
![Western Blot: MTHFR Antibody [NBP2-27549]](https://resources.bio-techne.com/images/products/MTHFR-Antibody-Western-Blot-NBP2-27549-img0001.jpg "Western Blot: MTHFR Antibody [NBP2-27549]")
Western Blot: MTHFR Antibody [NBP2-27549]
Western Blot: MTHFR Antibody [NBP2-27549] - (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for MTHFR Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in NIH3T3 lysates (calculated MW of 74.6 kDa band according to human NP_005948.3 and mouse NP_034970.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MTHFR
Alternate Names
EC 1.5.1.20, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH)
Gene Symbol
MTHFR
UniProt
Additional MTHFR Products
Product Documents for MTHFR Antibody
Product Specific Notices for MTHFR Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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