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mtRNA polymerase Antibody (3D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005442-M01

Novus Biologicals, part of Bio-Techne
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H00005442-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

POLRMT (NP_005026, 1121 a.a. ~ 1230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

Specificity

POLRMT - polymerase (RNA) mitochondrial (DNA directed) (3D9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for mtRNA polymerase Antibody (3D9)

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody (3D9) [H00005442-M01]

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody (3D9) [H00005442-M01]

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody (3D9) [H00005442-M01] - Analysis of monoclonal antibody to POLRMT on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: mtRNA polymerase Antibody (3D9) [H00005442-M01] - Detection limit for recombinant GST tagged POLRMT is 0.3 ng/ml as a capture antibody.

Applications for mtRNA polymerase Antibody (3D9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: mtRNA polymerase

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq]

Long Name

DNA-directed RNA polymerase, mitochondrial

Alternate Names

EC 2.7.7.6, MtRPOL, POLRMT

Entrez Gene IDs

5442 (Human)

Gene Symbol

POLRMT

Additional mtRNA polymerase Products

Product Documents for mtRNA polymerase Antibody (3D9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mtRNA polymerase Antibody (3D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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