mtRNA polymerase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90126

Novus Biologicals, part of Bio-Techne
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NBP1-90126
NBP1-90126-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all mtRNA polymerase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLH

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23070475)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mtRNA polymerase Antibody

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody [NBP1-90126]

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody [NBP1-90126]

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP1-90126] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126]

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126]

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human skeletal muscle shows strong granular cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126]

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126]

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.
View 2 more images

Applications for mtRNA polymerase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mtRNA polymerase

POLRMT, also known as DNA-directed RNA polymerase, mitochondrial, is a 246 amino acid protein that is 28 kDa, catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. This protein is currently being studied for research on autosomal dominant progressive external ophthalmoplegia, essential thrombocythemia, ophthalmoplegia, immunodeficiency, and malaria. This protein has also shown to have interactions with TFB1M, NFKB2, ICT1, TFB2M, TFAM, and over 200 other proteins in ATP/ITP metabolism, mitochondrial gene expression, transcription from mitochondrial promoters, transcription, RNA polymerase I, RNA polymerase III, and mitochondrial transcription, gene expression, and mitochondrial transcription initiation pathways.

Long Name

DNA-directed RNA polymerase, mitochondrial

Alternate Names

EC 2.7.7.6, MtRPOL, POLRMT

Entrez Gene IDs

5442 (Human)

Gene Symbol

POLRMT

UniProt

O00411 (Human)

Additional mtRNA polymerase Products

Product Documents for mtRNA polymerase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for mtRNA polymerase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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