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mtTFA Antibody (S08-3E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19504

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19504-100ul
NBP3-19504-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-3E2

Concentration

0.3 mg/ml

Product Summary for mtTFA Antibody (S08-3E2)

Immunogen

A synthetic peptide of human mtTFA (Uniprot # Q00059)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mtTFA Antibody (S08-3E2)

Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504]

Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504]

Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504]

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504]

Immunocytochemistry/Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunofluorescence of mtTFA (green) in Hela cells using NBP3-19504 at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504]

Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504]

Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using NBP3-19504 at dilution 1/1

Applications for mtTFA Antibody (S08-3E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Immunohistochemistry

1/50

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mtTFA

TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Alternate Names

mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial

Gene Symbol

TFAM

Additional mtTFA Products

Product Documents for mtTFA Antibody (S08-3E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mtTFA Antibody (S08-3E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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