MUC1 Antibody (rMUC1/960)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53286
![Western Blot: MUC1 Antibody (rMUC1/960) [NBP2-53286]](https://resources.bio-techne.com/images/products/MUC1-Antibody-rMUC1-960-Western-Blot-NBP2-53286-img0002.jpg "Western Blot: MUC1 Antibody (rMUC1/960) [NBP2-53286]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # rMUC1/960
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human MUC1 protein (Uniprot: P15941 )
Localization
Cytoplasmic and cell surface
Specificity
This monoclonal antibody reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this monoclonal antibody involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
200ug/ml of recombinant MAb purified by Protein A or G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. (NBP2-54346)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MUC1 Antibody (rMUC1/960)
Western Blot: MUC1 Antibody (rMUC1/960) [NBP2-53286]
Western Blot: MUC1 Antibody (rMUC1/960) [NBP2-53286] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).![Immunohistochemistry-Paraffin: MUC1 Antibody (rMUC1/960) [NBP2-53286]](https://resources.bio-techne.com/images/products/MUC1-Antibody-rMUC1-960-Immunohistochemistry-Paraffin-NBP2-53286-img0001.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (rMUC1/960) [NBP2-53286]")
Immunohistochemistry-Paraffin: MUC1 Antibody (rMUC1/960) [NBP2-53286]
Immunohistochemistry-Paraffin: MUC1 Antibody (rMUC1/960) [NBP2-53286] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMA Recombinant Mouse Monoclonal Antibody (MUC1/960).Applications for MUC1 Antibody (rMUC1/960)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MUC-1
Overexpression of mucins, including MUC1, is a feature of many epithelial cancers (1,3,5,6). The presence of truncated glycan structures called tumor-associated carbohydrate antigens (TACAs) on MUC1 play a role in cancer progression and a loss of apical-basal polarity (5). Carbohydrate-binding partners called lectins are the primary binding partners of TACAs that give rise to the pro-tumor microenvironment and metastasis (5). Given this unique feature, TACAs are a potential target for cancer immunotherapies (5). There are a number of vaccines, drugs, and antibodies targeting MUC1 for treatment of a variety of cancers including breast, lung, and prostate (6). In addition to a role in cancer progression, MUC1, and specifically the CT portion, has been shown to have a positive, anti-inflammatory role in a variety of lung and airway infections (7).
References
1. Khodabakhsh, F., Merikhian, P., Eisavand, M. R., & Farahmand, L. (2021). Crosstalk between MUC1 and VEGF in angiogenesis and metastasis: a review highlighting roles of the MUC1 with an emphasis on metastatic and angiogenic signaling. Cancer cell international. https://doi.org/10.1186/s12935-021-01899-8
2. Nath, S., & Mukherjee, P. (2014). MUC1: a multifaceted oncoprotein with a key role in cancer progression. Trends in molecular medicine. https://doi.org/10.1016/j.molmed.2014.02.007
3. Dhar, P., & McAuley, J. (2019). The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2019.00117
4. Uniprot (P15941)
5. Beckwith, D. M., & Cudic, M. (2020). Tumor-associated O-glycans of MUC1: Carriers of the glyco-code and targets for cancer vaccine design. Seminars in immunology. https://doi.org/10.1016/j.smim.2020.101389
6. Almasmoum H. (2021). The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer. Cancer management and research. https://doi.org/10.2147/CMAR.S299089
7. Ballester, B., Milara, J., & Cortijo, J. (2021). The role of mucin 1 in respiratory diseases. European respiratory review : an official journal of the European Respiratory Society. https://doi.org/10.1183/16000617.0149-2020
Long Name
Mucin 1, Cell Surface-associated
Alternate Names
CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT
Gene Symbol
MUC1
UniProt
Additional MUC-1 Products
Product Documents for MUC1 Antibody (rMUC1/960)
Product Specific Notices for MUC1 Antibody (rMUC1/960)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...