MUC4 Antibody (5J3R7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16186

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16186-100ul
NBP3-16186-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J3R7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MUC-4 Antibodies »

Product Summary for MUC4 Antibody (5J3R7)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4807-4916 of human MUC4 (Q99102). WATVSVIALSNILHASASLPPEYQNRTEGLLGVWNNNPEDDFRMPNGSTIPPGSPEEMLFHFGMTWQINGTGLLGKRNDQLPSNFTPVFYSQLQKNSSWAEHLISNCDGD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MUC4 Antibody (5J3R7)

Western Blot: MUC4 Antibody (5J3R7) [NBP3-16186]

Western Blot: MUC4 Antibody (5J3R7) [NBP3-16186]

Western Blot: MUC4 Antibody (5J3R7) [NBP3-16186] - Western blot analysis of extracts of HeLa cells, using MUC4 antibody (NBP3-16186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186] - Immunohistochemistry of paraffin-embedded human colon using MUC4 Rabbit mAb (NBP3-16186) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186] - Immunohistochemistry of paraffin-embedded rat kidney using MUC4 Rabbit mAb (NBP3-16186) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for MUC4 Antibody (5J3R7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MUC-4

MUC4 is a constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, and is a highly glycosylated proteins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 antibody studies have shown that MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 is expressed in a wide variety of carcinomas making MUC4 antibodies a key cancer research tool.

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Gene Symbol

MUC4

Additional MUC-4 Products

Product Documents for MUC4 Antibody (5J3R7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MUC4 Antibody (5J3R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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