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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MUC4/3105

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa 1730-1864) of human MUC4 protein (exact sequence is proprietary) (Uniprot: Q99102)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

235 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79867)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MUC4 Antibody (MUC4/3105)

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC4 Antibody (MUC4/3105).
Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC4 Antibody (MUC4/3105).
Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732]

Immunohistochemistry-Paraffin: MUC4 Antibody (MUC4/3105) [NBP2-79732] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC4 Antibody (MUC4/3105).

Applications for MUC4 Antibody (MUC4/3105)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MUC-4

MUC4 is a constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, and is a highly glycosylated proteins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 antibody studies have shown that MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 is expressed in a wide variety of carcinomas making MUC4 antibodies a key cancer research tool.

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Gene Symbol

MUC4

UniProt

Additional MUC-4 Products

Product Documents for MUC4 Antibody (MUC4/3105)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MUC4 Antibody (MUC4/3105)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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