MUC5AC Antibody (7E9D8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61875

Novus Biologicals, part of Bio-Techne
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NBP2-61875
NBP2-61875-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7E9D8

Format

BSA Free

Concentration

1 mg/ml

View all MUC5AC Antibodies »

Product Summary for MUC5AC Antibody (7E9D8) - BSA Free

Immunogen

Purified recombinant fragment of human MUC5AC (AA: 5528-5627) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

585 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MUC5AC Antibody (7E9D8) - BSA Free

Western Blot: MUC5AC Antibody (7E9D8)BSA Free [NBP2-61875]

Western Blot: MUC5AC Antibody (7E9D8)BSA Free [NBP2-61875]

Western Blot: Mucin 5AC Antibody (7E9D8) [NBP2-61875] - Analysis using MUC5AC mAb against human MUC5AC (AA: 5528-5627) recombinant protein. (Expected MW is 37 kDa)
Flow Cytometry: MUC5AC Antibody (7E9D8) - BSA Free [NBP2-61875]

Flow Cytometry: MUC5AC Antibody (7E9D8) - BSA Free [NBP2-61875]

Flow Cytometry: Mucin 5AC Antibody (7E9D8) [NBP2-61875] - Analysis of Hela cells using MUC5AC mouse mAb (green) and negative control (red).
Western Blot: MUC5AC Antibody (7E9D8)BSA Free [NBP2-61875]

Western Blot: MUC5AC Antibody (7E9D8)BSA Free [NBP2-61875]

Western Blot: Mucin 5AC Antibody (7E9D8) [NBP2-61875] - Analysis using MUC5AC mAb against HEK293 (1) and MUC5AC (AA: 5528-5627)-hIgGFc transfected HEK293 (2) cell lysate.
View 1 more images

Applications for MUC5AC Antibody (7E9D8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MUC5AC

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Alternate Names

gastric mucin, leB, lewis B blood group antigen, major airway glycoprotein, MUC5, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, mucin-5AC, TBM, tracheobronchial mucin

Gene Symbol

MUC5AC

Additional MUC5AC Products

Product Documents for MUC5AC Antibody (7E9D8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MUC5AC Antibody (7E9D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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