Key Product Details

Species Reactivity

Human, Mouse (Negative), Primate

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 19.4E

Format

BSA Free

Concentration

1 mg/ml

View all MUC5B Antibodies »

Product Specifications

Immunogen

Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B.

Specificity

Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Relevant Entrez Gene Numbers: NM_002458.1

Applications for MUC5B Antibody (19.4E) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Western Blot; Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins. Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Liquid in 0.02M Phosphate buffer, 0.25 M NaCl.

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MUC5B

Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 10E3 kDa.

Alternate Names

high molecular weight salivary mucin MG1, mucin 5, subtype B, tracheobronchial, mucin 5B, oligomeric mucus/gel-forming, sublingual gland mucin, tracheobronchial

Gene Symbol

MUC5B

UniProt

Q9HC84 (Human)

Additional MUC5B Products

Product Documents for MUC5B Antibody (19.4E) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MUC5B Antibody (19.4E) - BSA Free

Relevant Entrez Gene Numbers: NM_002458.1

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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