MURC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86609

Novus Biologicals, part of Bio-Techne
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NBP1-86609
NBP1-86609-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MURC Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KGKDRTVAEGEECAREMGVDIIARSESLGPISELYSDELSEPEHEAARPVYPPHEGREIPTPEPLKVTFKSQVKVEDDESLLL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26086601).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MURC Antibody

Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609]

Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609]

Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining in human skeletal muscle and pancreas tissues using anti-MURC antibody. Corresponding MURC RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609]

Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609]

Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human cerebral cortex, colon, pancreas and skeletal muscle using Anti-MURC antibody NBP1-86609 (A) shows similar protein distribution across tissues to independent antibody NBP1-86608 (B).
Western Blot: MURC Antibody [NBP1-86609]

Western Blot: MURC Antibody [NBP1-86609]

Western Blot: MURC Antibody [NBP1-86609] - Analysis in control (vector only transfected HEK293T lysate) and MURC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for MURC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MURC

Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCKsignaling pathway

Alternate Names

muscle-related coiled-coil protein, Muscle-restricted coiled-coil protein

Gene Symbol

MURC

Additional MURC Products

Product Documents for MURC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MURC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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