MuRF1/TRIM63 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1193

Novus Biologicals, part of Bio-Techne
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NB100-1193
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MuRF1/TRIM63 Antibodies »

Product Specifications

Immunogen

Peptide with sequence DYKSSLIQDGNPM-C corresponding to N-Terminus according to NP_115977.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MuRF1/TRIM63 Antibody

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193]

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193]

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193] - (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MuRF1/TRIM63 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:32000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human skeletal muscle lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2 kDa band according to NP_115977.2. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human skeletal muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MuRF1/TRIM63

This protein is a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Long Name

Tripartite Motif-containing 63

Alternate Names

IRF, RNF28, SMRZ, TRIM63

Entrez Gene IDs

84676 (Human)

Gene Symbol

TRIM63

UniProt

NP_115977 (Human)

Additional MuRF1/TRIM63 Products

Product Documents for MuRF1/TRIM63 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MuRF1/TRIM63 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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