MuRF1/TRIM63 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23425
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MuRF1/TRIM63 Antibody
Immunogen
MuRF1/TRIM63 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1/TRIM63. This peptide sequence is highly conserved in rat and mouse MuRF1/TRIM63, and has 50% homology to MuRF2 (TRIM-55). [UniProt# Q969Q1]
Specificity
The antibody detects 38 and 30 kDa proteins corresponding to the apparent molecular mass of MuRF1/TRIM63 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for MuRF1/TRIM63 Antibody
Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] -
Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.
Western Blot: MuRF1/TRIM63 Antibody [NBP3-23425] -
Western Blot: MuRF1/TRIM63 Antibody [NBP3-23425] - Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1/TRIM63 (C-terminal region) (lanes 1 & 2) or anti-Fbx32 (lanes 3 & 4).Applications for MuRF1/TRIM63 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: MuRF1/TRIM63
Long Name
Tripartite Motif-containing 63
Alternate Names
IRF, RNF28, SMRZ, TRIM63
Gene Symbol
TRIM63
Additional MuRF1/TRIM63 Products
Product Documents for MuRF1/TRIM63 Antibody
Product Specific Notices for MuRF1/TRIM63 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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