Musashi-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52978
![Western Blot: Musashi-2 AntibodyBSA Free [NBP2-52978]](https://resources.bio-techne.com/images/products/Musashi-2-Antibody-Western-Blot-NBP2-52978-img0003.jpg "Western Blot: Musashi-2 AntibodyBSA Free [NBP2-52978]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Full length recombinant human Mushashi 2 protein [UniProt B4DHE8]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Musashi-2 Antibody - BSA Free
Western Blot: Musashi-2 AntibodyBSA Free [NBP2-52978]
Western Blot: Musashi-2 Antibody [NBP2-52978] - Total protein from HeLa, Neuro2A and SH-SY5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MS12 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody - BSA Free [NBP2-52978]](https://resources.bio-techne.com/images/products/Musashi-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52978-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody - BSA Free [NBP2-52978]")
Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody - BSA Free [NBP2-52978]
Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP2-52978] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MSI2 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: Musashi-2 Antibody - BSA Free [NBP2-52978]](https://resources.bio-techne.com/images/products/Musashi-2-Antibody-Immunohistochemistry-Paraffin-NBP2-52978-img0002.jpg "Immunohistochemistry-Paraffin: Musashi-2 Antibody - BSA Free [NBP2-52978]")
Immunohistochemistry-Paraffin: Musashi-2 Antibody - BSA Free [NBP2-52978]
Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-52978] - IHC analysis of a formalin fixed paraffin embedded tissue section of murine pancreas using rabbit anti-Musashi-2 antibody at 1:400 dilution. The signal was developed using HRP-DAB based detection method and the counter-staining was performed with hematoxylin. The antibody generated a weaker signal in the exocrine glandular cells (acini) with very strong staining in the Islets of Langerhans, especially in the alpha cells.Applications for Musashi-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Musashi-2
Additional Musashi-2 Products
Product Documents for Musashi-2 Antibody - BSA Free
Product Specific Notices for Musashi-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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