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Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03052

Novus Biologicals, part of Bio-Techne
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NBP3-03052

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 261-319 of human Muscarinic Acetylcholine Receptor M4/CHRM4 (NP_000732.2). EAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Muscarinic Acetylcholine Receptor M4/CHRM4 AntibodyAzide and BSA Free [NBP3-03052]

Western Blot: Muscarinic Acetylcholine Receptor M4/CHRM4 AntibodyAzide and BSA Free [NBP3-03052]

Western Blot: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NBP3-03052] - Analysis of extracts of NIH/3T3 cells, using Muscarinic Acetylcholine Receptor M4/CHRM4 antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHRM4

Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.

Long Name

Cholinergic Receptor Muscarinic 4

Alternate Names

HM4, M4, M4R, mAChR M4

Gene Symbol

CHRM4

Additional CHRM4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov