MVP Antibody (2H3-1A6)

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01]

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - MVP monoclonal antibody (M01), clone 2H3-1A6 Analysis of MVP expression in Hela S3 NE.

ELISA: MVP Antibody (2H3-1A6) [H00009961-M01]

ELISA: MVP Antibody (2H3-1A6) [H00009961-M01] - Detection limit for recombinant GST tagged MVP is approximately 1ng/ml as a capture antibody.

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] -

Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Immunoblot validation & characterization of dysferlin.A) Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 & 2 (SPTLC1 & SPTLC2), HLA-ABC, & intercellular adhesion molecule 4 (ICAM4) with beta-actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. B) Confocal images of intact RBCs from DBA patient D1 & healthy control C2 showing localization of dysferlin (green) at the membrane where spectrin (red) is localized. Dysferlin fluorescence signal in the membrane of DBA RBCs is present in punctuate patterns & is evident above the autofluorescent background seen in the cytoplasm as highlighted in the zoomed images on the right panel. C) Western blot showing the enrichment of dysferlin in the RBC ghosts (WG) & the inside-out vesicles (IOV) but not in the RBC membrane cytoskeletal extract (CE) of DBA patients D1 & D2. D) Distribution of dysferlin spectral counts versus gel slice number for DBA samples D1 & D2. Higher abundance of dysferlin in D1 with high molecular weight dysferlin crosslinks & low mass protein fragments correlate with immunoblots shown in panel A. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24454878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.