MYBBP1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61050

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Cited:

CoIP, Immunoprecipitation, Proximity Ligation Assay, RIP-ChIP, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MYBBP1A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human MYB binding protein (P160) 1a using the numbering given in entry NP_055335.1 (GeneID 10514).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYBBP1A Antibody

Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050]

Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050]

Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-MYBBP1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MYBBP1A Antibody [NB100-61050]

Immunoprecipitation: MYBBP1A Antibody [NB100-61050]

Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of human MYBBP1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MYBBP1A antibody NB100-61050 used for IP at 3 ug per reaction. MYBBP1A was also immunoprecipitated by rabbit anti-MYBBP1A antibody NB100-61051. For blotting immunoprecipitated MYBBP1A, NB100-61051 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for MYBBP1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Use in Western blot and proximity ligation assay reported in scientific literature (PMID 25303791). Use in Proximity Ligation Assay reported in scientific literature (PMID: 25303791). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MYBBP1A

Myb binding protein 1a (MYBBP1a) was originally isolated as a c-Myb interacting protein but has been found to associate with a number of transcription factors. MYBBP1a has been shown to activate or repress transcription through its interaction with transcriptional regulators such as AHR, jun, c-Myb, NF-kappaB, and PGC-1alpha.

Alternate Names

MYB binding protein (P160) 1a, myb-binding protein 1A, P160FLJ37886, p53-activated protein-2, PAP2

Entrez Gene IDs

10514 (Human)

Gene Symbol

MYBBP1A

UniProt

NP_055335.1 (Human)

Additional MYBBP1A Products

Product Documents for MYBBP1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MYBBP1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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